Table of Contents
Study Materials
Readings
MATLAB® software is required to run the .m files in this section.
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Suggested Readings in Biology
The following sections from Alberts, Bruce, et al. Molecular Biology of the Cell. 4th ed. New York: Garland Science, 2002. ISBN: 9780815332183, are suggested:
- RNA and Protein Synthesis
- Overview of Gene Control
- How Genetic Switches Work
- General Principles of Cell Signaling
| LEC # | TOPICS | READINGS |
|---|---|---|
| Part I: Systems Microbiology - 'The Cell as a Well-stirred Bioreactor' | ||
| 1 | Introduction Michaelis-Menten Kinetics | |
| 2 | Equilibrium Binding Cooperativity | Michaelis-Menten Kinetics (PDF) Associated MATLAB® code file (M) |
| 3 | Lambda Phage Multistability | A Genetic Switch in Lamba Phage (PDF) Associated MATLAB® code file (M) Hasty, Jeff, Joel Pradines, Milos Dolnik, and J. J. Collins. "Noise-based Switches and Amplifiers for Gene Expression." Proc. Natl. Acad. Sci. USA 97, no. 5 (Feb 29, 2000): 2075-80. |
| 4 | Multistability (cont.) | Isaacs, Farren J., Jeff Hasty, Charles R. Cantor, and J. J. Collins. "Prediction and Measurement of an Autoregulatory Genetic Module." PNAS 100, no. 13 (June 24, 2003): 7714-19. |
| 5 | Synthetic Genetic Switches | Synthetic Genetic Switches (PDF) Gardner, Timothy S., Charles R. Cantor, and James J. Collins. "Construction of a Genetic Toggle Switch in Escherichia coli." Nature 403, no. 6767 (January 20, 2000): 339-42. |
| 6 | Stability Analysis | Stability Analysis (PDF) |
| 7 | Introduction E. coli Chemotaxis | |
| 8 | Fine-tuned versus Robust Chemotaxis Models | Modeling Escherichia coli chemotaxis (PDF) Associated MATLAB® code file (M) Spiro, Peter A., John S. Parkinson, and Hans G. Othmer. "A Model of Excitation and Adaptation in Bacterial Chemotaxis." Proc. Natl. Acad. Sci. USA 94, no. 14 (July, 1997): 7263–68. |
| 9 | Wrapping up Chemotaxis | Biological Oscillators (PDF) |
| 10 | Genetic Oscillators | Biological Oscillators (PDF) Associated MATLAB® code file (M) Elowitz, Michael B., and Stanislas Leibler. "A Synthetic Oscillatory Network of Transcriptional Regulators." Nature 403, no. 6767 (January 20, 2000): 335-8. |
| 11 | Genetic Oscillators (cont.) | Routh-Hurwitz (PDF) Associated MATLAB® code file (M) Atkinson, Mariette R., Michael A. Savageau, Jesse T. Myers, and Alexander J. Ninfa. "Development of Genetic Circuitry Exhibiting Toggle Switch or Oscillatory Behavior in Escherichia coli." Cell 113, no. 5 (May 30, 2003): 597-607. |
| 12 | Stochastic Chemical Kinetics | The Origin and Consequences of Noise in Biochemical Systems (PDF) (Courtesy of Alexander van Oudenaarden and Mukund Thattai. Used with permission.) |
| 13 | Stochastic Chemical Kinetics (cont.) | |
| Part II: Cell Systems Biology - 'The Importance of Diffusion and Gradients for Cellular Regulation' | ||
| 14 | Introduction Cell Systems Biology Fick's Laws | |
| 15 | Local Excitation Global Inhibition Theory | Local Excitation, Global Inhibition Model (PDF) |
| 16 | Local Excitation (cont.) Global Inhibition Theory (cont.) | |
| 17 | Rapid Pole-to-pole Oscillations in E. coli | Howard, Martin, Andrew D. Rutenberg, and Simon de Vet. "Dynamic Compartmentalization of Bacteria: Accurate Division in E. Coli." Physical Review Letters 87, no. 27 (December 31, 2001). |
| 18 | Rapid Pole-to-pole Oscillations in E. coli (cont.) | |
| 19 | Models for Eukaryotic Gradient Sensing | Models for Eukaryotic Gradient Sensing (PDF) Associated MATLAB® code file (M) Narang, Atul, K. K. Subramanian, and D. A. Lauffenburger. "A Mathematical Model for Chemoattractant Gradient Sensing based on Receptor-regulated Membrane Phospholipid Signaling Dynamics." Annals of Biomedical Engineering 29, no. 8 (2001): 677-91. |
| 20 | Models for Eukaryotic Gradient Sensing (cont.) | Postma, Marten, and Peter J. M. Van Haastert. "A Diffusion–Translocation Model for Gradient Sensing by Chemotactic Cells." Biophysical Journal 81, no. 3 (September, 2001): 1314-23. |
| 21 | Modeling Cytoskeleton Dynamics | Dogterom, Marileen, and Stanislas Leibler. "Physical Aspects of the Growth and Regulation of Microtubule Structures." Physical Review Letters 70, no. 9 (March 1, 1993). |
| 22 | Modeling Cytoskeleton Dynamics (cont.) | Cytrynbaum, E. N., V. Rodionov, and A. Mogilner. "Computational Model of Dynein-dependent Self-organization of Microtubule Asters." Journal of Cell Science 117, no. 8 (March 15, 2004): 1381-97. |
| Part III: Developmental Systems Biology - 'Building an Organism Starting From a Single Cell' | ||
| 23 | Quorum Sensing | You, Lingchong, Robert Sidney Cox III, Ron Weiss, and Frances H. Arnold. "Programmed Population Control by Cell-Cell Communication and Regulated Killing." Nature 428, no. 6985 (April 22, 2004): 868-71. |
| 24 | Final Problem Set Question Hour | |
| 25 | Drosophila Development | Houchmandzadeh, Bahram, Eric Wieschaus, and Stanislas Leibler. "Establishment of Developmental Precision and Proportions in the Early Drosophila embryo." Nature 415, no. 6873 (February 14, 2002): 798-802. |